Lyophilized Exosome Standards

The purest lyophilized standards from human biofluids and different cell culture media.

Lyophilization is a useful technique that can be used for long-term stable preservation of exosomes. Besides, lyophilized exosome can be used as control standards for FACS, WB, ELISA and other applications, as well as quantitative calibration standards for foreign and domestic exosomes in biological samples.

Immunostep Lyophilized exosome standards can be used as control standards for multiple applications, as calibration standards for biomarkers from biological samples and for immunocapture performance evaluation.

High pure exosomes improving performance over competitors

Purity validated by WB, NTA, Cytometry and functional analysis in vitro.

Tested and validated for regenerative medicine, skin, dermatological and pharma companies.

The purest lyophilized Exosome standards

Lyophilized exosome standards easy to transport and storage and is stable for over 2 years at 2°C to 8ºC.  Immunostep provides the highest quality lyophilized exosome standards isolated from a variety of biological sources, including cell culture supernatant, human plasma, serum, urine, and saliva. Exosomes are isolated by differential ultracentrifugation.

Biofluids or Cell Culture Media

Sample Collection

Purification via ultracentrifuge

Lyophilization and Quantification

Guaranteed performance validated by WB, NTA, Cytometry and functional analysis in vitro.

All exosome standard batches are validated using FCM, WB and NTA Analysis. Additionally, in order to compare the effects of lyophilization process we compare all lyophilized batches with respect to fresh exosomes stored at -20ºC. Dynamic range of fresh and lyophilized PC3 exosomes are analysed by flow cytometry

Figure 1: Relationship between background noise and specific signal at different exosome concentrations. Exosomes were captured by CD63+ (CloneTEA3/18) capture beads and subsequently detected by Anti-CD9PE (CloneVJ1/20).

We are looking forward to hearing about your research

Batch Validation: High quality and pure standards

For its validations we carry out an exosome analysis and comparative of fresh  and lyophilized plasma exosomes for particle size and concentration by NTA, NanoSightLM10HSB. This analysis is carried out with 1 μl of purified exosomes diluted in 999 μL of HEPES buffer (dilution1:1000). The purified exosomes show  a size distribution profiles, with peak diameters from 50 –150 nm and concentrations about 1x1010exosomes/ml. Additionally, fresh, and lyophilized MCF-7 exosome batches are analysed and compared by WB in native conditions for exosomal markers, by anti-CD9 (Clone VJ1/20) and anti-CD63 (Clon TEA3/18) antibodies at a 1:1000 dilution (0,1 mg/m).

References

Kit NameKit Description
Exosomes From PC-3

Human metastatic prostate cancer cell line | ExoPC3  | 100µl

Get a quote
Exosomes From HT-29

Human metastatic colon cancer cell line | ExoHT29  | 100µl

Get a quote
Exosomes From MCF-7

Human metastatic prostate breast cell line | ExoMCF7  | 100µl

Get a quote
Exosomes From A-375

Human malignant melanoma cell line | ExoA375  | 100µl

Get a quote
MSCs Derived Exosomes

Adipose-derived Mesenchymal stem/stromal Cells (MSCs) derived exosomes| ExoMSC  | 100µl

Get a quote
Exosomes from RPMI

RPMI Cell Line | ExosRPMI | 100μg

GET A QUOTE
Exosomes From CaCo2

A human colon cancer cell line | ExoCaCo2 | 100μg 

GET A QUOTE

You might be also  interested in:

  • Human Exosomal Antibody Markers 

    Customize Antibodies available for the detection of specific exosomes.

  • ExoStep Kits

    Discover this powerful method to detect exosomes in small samples by flow cytometry.

  • Exosome precipitation solutions

    Exosome precipitation solutions to combine with ExoStepTM kit

    Would you like to have a conversation about your EVs Research?

    We are looking forward to starting a conversation in order to support you through your research in any part of the world!




      Choose your interests:

      I have read the Privacy Policy and accept the terms.
      I would like to recive occasional Exosome News and Updates.

      TO TOP