ExoStep  Platform: Detection and characterization of Exosomes

ExoStepTM KIT: The most accurate method of exosome detection developed to date.

ExoStepTM kit is   the most accurate method of exosome isolation developed to date, a superior alternative for the sensitive detection of exosomes compared with the most commonly used methods besides being easy to implement and analyse for any laboratory that has access to a conventional flow cytometer.

Specific Exosome Detection in Biological Fluids by Flow Cytometry.

Wide Dynamic Range and Limit of Detection

Allowing Simultaneous Immunophenotyping of Exosomes Capture Population.

Easy to Perform Immunobead Assay with Quantitative and Scalable Results.

Specific and unambiguous exosome detection in biological fluids by flow cytometry:

ExoStepTM kit is an immunobead assay for the detection of exosomes using a bead-bound capture antibody and fluorochrome conjugated detection antibody. This kit provides reproducible results and is intended for flow cytometry analysis of pre-enriched human exosomes from biofluids (plasma, serum, urine, among others) or cell culture media.

Ultracentrifugation is not needed

Reproducible Results

Effective with Small Sample Quantities

 Bead-based Assay for Flow cytometry.

ExoStepTM  Kit for the detection and characterization of exosomes by flow cytometry is composed of an exosome capture reagent consisting of 6 µm diameter magnetic beads coated with a specific antibody  as solid suport detectable by the cytometer. Additionally, the capture beads are dyed red and fluorescently labelled which facilitate their detection in the fluorescent detector of a conventional flow cytometer. ExoStepTM contains a detector ab fluorescent conjugated for exosome detection.

*ExoELISA step Kit: ELISA Format Now Available

We are looking forward to hearing about your research

Wide Dynamic Range and limit of Detection.

To determine the dynamic range and limit of detection of the assay, EV titration experiments were performed using CD63 for capture and CD9 for detection. The sensitivity of the assay has demonstrated to be very high with a positive signal detected as little as thirty ng of exosomes while 2 ug were required for WB detection. Besides, flow cytometry data follows a linear distribution between 30 and 8 micrograms of exosomes, allowing interpolating fluorescent intensity values to estimate the amount of exosomes in the sample.

We work to apply our technology in the detection and quantification of disease biomarkers present in the surface of EVs as well as in diagnostic of diseases.

*This Technology is under patent licence: PET/EP2021/068633 “Method for the detection and/or quantification of extracellular vesicles in fluid biological samples”

Simultaneous Immunophenotyping 

Considering exosome direct detection results in body fluids, we want to anticipate researcher needs for exosome subpopulations characterization through specific markers. In order to optimize the protocol, we performed an immunophenotyping assay simultaneously with the exosome detection. This optimization of the protocol made possible to include in the FL2 channel (the most sensitive), the antibody against the marker of Interest. The suitability of the kit for the specific detection of exosomes was confirmed, making possible to further characterize their phenotype by labelling with other markers of interest

ExoELISA-Step Kit

ExoELISA-Step is an enzyme immunoassay for the detection and quantification of exosomes, based on the use of antigens or antibodies labeled with an enzyme, so that the resulting conjugates have both immunological and enzymatic activity. As one of the components (antigen or antibody) is labeled with an enzyme and insolubilized on a support (immunosorbent) the antigen-antibody reaction will remain immobilized and therefore, is easily revealed by the addition of a specific substrate. When acting on the enzyme a simple or quantifiable view will produce an observable color using a spectrophotometer or a colorimeter.

References

Kit NameKit Description+info
ExoELISA-step Kit

96 test |  Cell culture Sample: Exo2506 |  |Serum/plasma Sample: Exo2508 | ELISA Assays for detection and quantification of exosomes | Get a quote

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ExoStep culture

25 test | ExoS-25-C9 | Cell culture Sample | Superparamagnetic Capture Beads (CD63), Primary detection antibody (CD9 PE) and Assay Buffer 10X | Get a quote

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Exostep Plasma

25 test | ExoS-25-P81 | Plasma Samples | Superparamagnetic Capture Beads (CD9 Capture Beads) Primary detection antibody (CD81 PE) Assay Buffer 10X | Get a quote

Download TDS
Exostep Urine

25 test | ExoS-25-U9 | Urine Samples | Superparamagnetic Capture Beads (CD63 Capture Beads) Primary detection antibody (CD9 PE) Assay Buffer 10X.| Get a quote

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Exostep Culture + Standard

50 test | ExoS-50-CST9 | Cell culture Sample | Superparamagnetic Capture Beads (CD63 Capture Beads) Primary detection antibody (CD9 PE) Assay Buffer 10X Lyophilized exosomes (1×10^12) from PC-3 Human prostate cancer.| Get a quote

Download TDS
Exostep Plasma + Standard

50 test | ExoS-50-PST81 | Plasma Sample | Superparamagnetic Capture Beads (CD9 Capture Beads) Primary detection antibody (CD81 PE) Assay Buffer 10X Lyophilized exosomes (1×10^12) from Human Plasma.| Get a quote

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Exostep General Kit

50 test ● 25 test | ExoS-50-G9 ●   ExoS-50-G81 ●  ExoS-25-G9 ●  ExoS-25-G81 |Any biological Sample | Superparamagnetic Capture Beads (CD63 Capture Beads) Primary detection antibody (CD9 Biotin) or (CD81 Biotin) Secondary detection reagent (PE Conjugated) Assay Buffer 10X.

Download TDS
Mouse Exostep Culture

25 test | MO2ExoS-25-C |RUO mouse exosome from Cell Culture Samples | Superparamagnetic Capture Beads (CD63 Capture Beads) Primary detection antibody (CD9 Biotin) Secondary detection reagent (PE Conjugated) Assay Buffer 10X.| Get a quote

Download TDS

You might be also  interested in:

  • ExoStep Mouse

     Monoclonal antibody intended for mouse exosome detection using flow cytometry included.

  • Size Exclusion Columns (SEC) 

    Combine our Size Exclusion Columns (SEC) with ExoStepTM to isolate and detect exosomes in small samples.

  • Detection Antibodies

    Monoclonal antibody intended for the exosome detection using flow cytometry.

    Would you like to have a conversation about your EVs Research?

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