ExoStep  Platform: Detection and characterization of Exosomes

ExoStepTM KIT: The most accurate method of exosome detection developed to date.

ExoStepTM kit is   the most accurate method of exosome isolation developed to date, a superior alternative for the sensitive detection of exosomes compared with the most commonly used methods besides being easy to implement and analyse for any laboratory that has access to a conventional flow cytometer.

Specific Exosome Detection in Biological Fluids by Flow Cytometry.

Wide Dynamic Range and Limit of Detection

Allowing Simultaneous Immunophenotyping of Exosomes Capture Population.

Easy to Perform Immunobead Assay with Quantitative and Scalable Results.

Specific and unambiguous exosome detection in biological fluids by flow cytometry:

ExoStepTM kit is an immunobead assay for the detection of exosomes using a bead-bound capture antibody and fluorochrome conjugated detection antibody. This kit provides reproducible results and is intended for flow cytometry analysis of pre-enriched human exosomes from biofluids (plasma, serum, urine, among others) or cell culture media.

Ultracentrifugation is not needed

Reproducible Results

Effective with Small Sample Quantities

 Bead-based Assay for Flow cytometry.

Exostep Platform for the detection and characterization of exosomes by flow cytometry is composed of an exosome capture reagent consisiting of 6 µm diameter magnetic beads coated with an specific antibody  as solid suport detectable by the cytometer. Additionally, the capture beads are dyed red and fluorescently labelled which facilitate their detection in the fluorescent detector of a conventional flow cytometer. ExoStepTM contains a detector ab fluoresct conjugated for exosome detection.

We are looking forward to hearing about your research

Wide Dynamic Range and limit of Detection.

To determine the dynamic range and limit of detection of the assay, EV titration experiments were performed using CD63 for capture and CD9 for detection. The sensitivity of the assay has demonstrated to be very high with a positive signal detected as little as thirty ng of exosomes while 2 ug were required for WB detection. Besides, flow cytometry data follows a linear distribution between 30 and 8 micrograms of exosomes, allowing interpolating fluorescent intensity values to estimate the amount of exosomes in the sample.

We work to apply our technology in the detection and quantification of disease biomarkers present in the surface of EVs as well as in diagnostic of diseases.

*This Technology is under patent licence: PET/EP2021/068633 “Method for the detection and/or quantification of extracellular vesicles in fluid biological samples”

Simultaneous Immunophenotyping

Considering exosome direct detection results in body fluids, we want to anticipate researcher needs for exosome subpopulations characterization through specific markers. In order to optimize the protocol, we performed an immunophenotyping assay simultaneously with the exosome detection. This optimization of the protocol made possible to include in the FL2 channel (the most sensitive), the antibody against the marker of Interest. The suitability of the kit for the specific detection of exosomes was confirmed, making possible to further characterize their phenotype by labelling with other markers of interest.

References

Kit NameKit Description+info
ExoStepTM culture

25 test | ExoS-25 | Cell culture Sample | Superparamagnetic Capture Beads (CD63), Primary detection antibody (CD9 PE) and Assay Buffer 10X

Download TDS
ExostepTM Plasma

25 test | ExoS-25-P81 | Plasma Samples | Superparamagnetic Capture Beads (CD63 Capture Beads) Primary detection antibody (CD9 PE) Assay Buffer 10X.

Download TDS
ExostepTM Urine

25 test | ExoS-25-U9 | Urine Samples | Superparamagnetic Capture Beads (CD63 Capture Beads) Primary detection antibody (CD9 PE) Assay Buffer 10X.

Download TDS
ExostepTM Culture + Standard

50 test | ExoS-50-CST9 | Cell culture Sample | Superparamagnetic Capture Beads (CD63 Capture Beads) Primary detection antibody (CD9 PE) Assay Buffer 10X Lyophilized exosomes (1×10^12) from PC-3 Human prostate cancer.

Download TDS
ExostepTM Plasma + Standard

50 test | ExoS-50-PST81 | Plasma Sample | Superparamagnetic Capture Beads (CD9 Capture Beads) Primary detection antibody (CD81 PE) Assay Buffer 10X Lyophilized exosomes (1×10^12) from Human Plasma.

Download TDS
ExostepTM General Kit

50 test | ExoS-50-G9/ExoS-25-G81 |Plasma or Urine Samples | Superparamagnetic Capture Beads (CD63 Capture Beads) Primary detection antibody (CD9 Biotin) or (CD81 Biotin) Secondary detection reagent (PE Conjugated) Assay Buffer 10X.

Download TDS

You might be also  interested in:

  • ExoStep Mouse

     Monoclonal antibody intended for mouse exosome detection using flow cytometry included.

  • Size Exclusion Columns (SEC) 

    Combine our Size Exclusion Columns (SEC) with ExoStepTM to isolate and detect exosomes in small samples.

  • Exosome Capture Beads

    Isolate specific exosomes among others, from biological fluids.

    Would you like to have a conversation about your EVs Research?

    We are looking forward to starting a conversation in order to support you through your research in any part of the world!




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