The biological characterization of exosomes requires in most cases the isolation of intact exosomes. In this sense, a large number o methods have been developed for the isolation of exosomes from biological fluids. In this sense, Immunostep offers some of the most common techniques for exosomes isolation with all the guarantees.
Thanks to our breakthrough technologies and years of experience in the field of exosomes, we are able to efficiently isolate high quality exosomes from a variety of cell types and multiple sources of biological fluids.
Isolation of EVs from a plasma by Immunostep SEC columns. Sample obtained can be subsequently analyzed by Abs (280) (Fig. A) and Abs (260/280) (Fig. B), which allows monitoring the elution of the sample, by conventional cytometry with the use of kits based on beads (Fig. C), such as Exostep (Ref. ExoS-25-P81).
A comparison was made using columns competitor, obtaining Immunostep columns a better performance in terms of recovery. A) Comparison of the diameter of the isolated particles by column type. The D50 value means 50% of the particles have that diameter or less. B) Concentration (particles / ml) obtained from purifying plasma (500 µl).
An adequate gating strategy FSC / SSC for 6 micron bead size and PerCP/APC, PerCP-Cy5/ APC or PerCP-Cy5.5/APC helps bead population identification and discrimination of doublets on flow cytometer. Have a look to this dot-plot gating strategy for acquisition and analysis FSC vs SSC (A) and PerCP vs APC (B).