The most suitable method to isolate exosomes from biological samples.

Size exclusion chromatography (SEC) has been described as most efficient method for isolating EVs from complex biological fluids in one single step, with a good recovery and with almost complete removal of contaminants, such as proteins and lipoproteins. Immunostep have developed 70nm and 35nm SEC columns for EVs isolation from complex biological fluids such as: plasma, serum, urine and cerebrospinal fluid.

Rapid & Reliable Exosome Purification

Reusable Columns and Efficient Packaging

Successfully separating different EVs sizes

 Great performance separating exosomes from important contaminants:

Immunostep reusable Size Exclusion Columns enable the rapid isolation of extracellular vesicles (EVs) from cell culture supernatants and complex biological fluids. Each column efficiently removes background proteins, lipids, solutes, and other contaminants to improve the sensitivity and accuracy of downstream assays (e.g., TRPS, protein profiling, RNA profiling, etc.) while maintaining the biological properties of the EVs. Vesicles are isolated in 15 minutes allowing EV samples to be collected and analysed immediately. Columns are available off-the-shelf, and quality assured so your isolations are reproducible from column to column.

Figure 1: (A) Amount of EVs and protein in each fraction from the column. Comparative of protein (BCA) vs vesicles (FACS CD63+/CD9+) content. (B) Flow cytometric Analysis of elution fractions. Stain Index = (MFI Positive-MFI background 2σ background) (C) Western Blot SEC fractions were loaded on SD-PAGE and immunoblotted for CD9 tetraspanin with anti-CD9 (VJ1/20), under no-reducing conditions.

We are looking forward to hearing about your research

 Successfully separating different EVs sizes

EVS SEC 35 NM
  •  Separation Size: 35-350nm

  • Sample Volume: <1ml (Optimal: 500 µl)

  • Volumetric Flow Rate at RT: >0.75ml/min

  • Column Volume: 10ml

EVS SEC 70 NM
  •  Separation Size: 70-1000nm

  • Sample Volume: <1ml (Optimal: 500 µl)

  • Volumetric Flow Rate at RT: >0.75ml/min

  • Column Volume: 10ml

Reusable columns and efficient packaging.

Immunostep columns have been developed to facilitate its execution, and for this reason it comes with a box which easily transform to a columns rack in the laboratory.

Figure 2. Graphic instructions to use column box as well as rack, avoiding to get a specific column rack in the laboratory and facilitating the protocol.

References

Kit NameKit Description
EVs SEC 70 nm – 4 pack

| 4 Columns | SEC7012-4

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EVs SEC 70 nm – 8 pack

| 8 Columns | SEC7012-8 | 

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EVs SEC 35 nm – 4 pack

| 4 Columns | SEC3512-4 | 

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EVs SEC 35 nm – 8 pack

| 8 Columns | SEC3512-8

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    Would you like to have a conversation about your EVs Research?

    We are looking forward to starting a conversation in order to support you through your research in any part of the world!




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