The most suitable method to isolate exosomes from biological samples.

Size exclusion chromatography (SEC) has been described as most efficient method for isolating EVs from complex biological fluids in one single step, with a good recovery and with almost complete removal of contaminants, such as proteins and lipoproteins. Immunostep have developed 70nm and 35nm SEC columns for EVs isolation from complex biological fluids such as: plasma, serum, urine and cerebral spinal fluid.

Rapid & Reliable Exosome Purification

Reusable columns and efficient packaging,

Successfully separating different EVs sizes

 Great performance separating exosomes from important contaminants:

Immunostep reusable Size Exclusion Columns enable the rapid isolation of extracellular vesicles (EVs) from cell culture supernatants and complex biological fluids. Each column efficiently removes background proteins, lipids, solutes, and other contaminants to improve the sensitivity and accuracy of downstream assays (e.g., TRPS, protein profiling, RNA profiling, etc.) while maintaining the biological properties of the EVs. Vesicles are isolated in 15 minutes allowing EV samples to be collected and analysed immediately. Columns are available off-the-shelf, and quality assured so your isolations are reproducible from column to column.

We are looking forward to hearing about your research

 Successfully separating different EVs sizes

EVS SEC 35 NM
  •  Separation Size: 35-350nm

  • Sample Volume: <1ml (Optimal: 500 µl)

  • Volumetric Flow Rate at RT: >0.75ml/min

  • Column Volume: 10ml

EVS SEC 70 NM
  •  Separation Size: 70-1000nm

  • Sample Volume: <1ml (Optimal: 500 µl)

  • Volumetric Flow Rate at RT: >0.75ml/min

  • Column Volume: 10ml

Reusable columns and efficient packaging.

Immunostep columns have been developed to facilitate its execution, and for this reason it comes with a box which easily transform to a columns rack in the laboratory.

Kit NameKit Description
EVs SEC 70 nm – 4 pack

| 4 Columns | SEC7012-4

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EVs SEC 70 nm – 8 pack

| 8 Columns | SEC7012-8 | 

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EVs SEC 35 nm – 4 pack

| 4 Columns | SEC3512-4 | 

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EVs SEC 35 nm – 8 pack

| 8 Columns | SEC3512-8

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Isolate Specific Exosomes From biological Fluids without previous Enrichment procedures:
  • Allowing Speicific Exosome Isolation From different Purification Techniques.

  • Direct Exosome Isolation Without previous Enrichment

  • Fully compatible with downstream analysis (WB, mRNA, miRNA…)

Product NameProduct Description
Human igG1 Capture Beads (Isotype Control)

for Flow Detection | IGG1CB-25 | 25 Test

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Human CD9 Capture Beads

for Flow Detection |9CB-25 | 25 Test

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Human CD63 Capture Beads

for Flow Detection | 63CB-25

 | 25 Test

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Human CD81 Capture Beads

for Flow Detection | 81CB-25 | 25 Test

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Human CD326 (EpCAM) Capture Beads

for Flow Detection | 326CB-25  | 25 Test

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Human CD274 (PD-L1) Capture Beads

for Flow Detection | 274CB-25 | 25 Test

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We are looking forward to hearing about your research

Easy and Rapid Precipitation Solutions: Increasing Biomarker Sensitivity detection
  • Ultracentrifugation Free method for the Highly- Sensitive Biomarker Detection.

  • Very  Clean Exosome Preparations: Reducing carry-over of Albumins and Immunoglobulins. 

  • Obtain Intact exosome suitable for a wide variety of protein-sensitive applications. 

Kit NameKit Description
Exosome Precipitation Solution

Cell Culture Media and Urine | EPStep | 12ml

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Exosome Precipitation Solution

Plasma and Serum | EPStep-PS| 5ml

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Exosome Precipitation Solution

Plasma+Trombin| EPStep-T | 5ml

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You might be also  interested in:

  • Lyophilized Exosome Standards

    The purest lyophilized standards from human biofluids and different cell culture media.

  • ExoStep Kits

    Combine our Size Exclusion Columns (SEC) with ExoStepTM to isolate and detect exosomes in small samples

  • Exosome precipitation solutions

    Intended for the extracellular vesicles (EVs) and specifically exosomes (50-150nm) from cell culture media and biofluids (plasma, serum, urine)

    Would you like to have a conversation about your EVs Research?

    We are looking forward to starting a conversation in order to support you through your research in any part of the world!




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