Immunostep’s isotypes controls IgG1b (clone B11/6) and IgG2a (clone B12_8) are monoclonal antibodies produced against a synthetic hapten, which is normally not present in humans or animals using in flow cytometry. These are often used as negative controls.
IgG1b is coated in the population beads number 1 while IgG2a is in population number 2. Both populations do not differ in size but in average fluorescence intensity.